By Patrick M. McCue, Edward L. Squires
This publication in brief studies the historical past of equine embryo move, protecting in clinically functional phrases the thoughts, gear, and administration protocols at present in use. Embryo move has develop into an incredible company, specifically for breeding racing inventory (horses and camels), and is hence an important element of equine perform. Ed Squires and Pat McCue were concerned with the improvement of embryo assortment and move techniques because the early 60s and feature either contributed very important recommendations and strategies to the method via their examine and scientific adventure. This booklet captures the scientific event, to this point, and applies it on to equine perform. The ebook is of significant price to normal equine practitioners for reference, equine replica experts, animal technological know-how on the graduate point (equine track), and breeders.
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Extra resources for Equine embryo transfer
Figure 6-6. “Open” system of embryo collection with “Y” tubing not directly attached to filter. 44 compared to the amount of fluid recovered to determine if a significant volume of fluid is still present in the uterus. Alternatively, the uterus may be examined by transrectal ultrasonography at the conclusion of the flush procedure to confirm that all of the media has been evacuated. Oxytocin (20 IU, intravenously) may be administered during the flush procedure to stimulate uterine contractions and aid in fluid recovery.
Castle Rock, Colorado). Unfortunately, at the present time, neither product is commercially available or approved for use in the horse. Recombinant Equine FSH A recombinant equine FSH (reFSH) preparation has been developed which is comprised of a single chain chimera with the amino terminus of the equine FSH alpha subunit and the carboxyl end of the equine FSH beta subunit attached to each other using the carboxyl terminal peptide of eCG/eLH beta. reFSH has been shown to have biological activity both in vitro and in vivo.
If the mare has been bred or inseminated, spermatozoa migrate proximally up the oviduct toward the newly ovulated oocyte(s) and fertilization occurs in the ampullary region of the oviduct. Although mares are usually bred with 500 million to several billion spermatozoa, only a few thousand sperm actually make it into the oviduct. Spermatozoa undergo a series of changes in the mare reproductive tract, a process called capacitation, in order to gain the ability to fertilize an oocyte. Capacitated sperm bind to specific proteins in the zona pellucida of the oocyte.
Equine embryo transfer by Patrick M. McCue, Edward L. Squires