By Christina E. Day
Histopathology: equipment and Protocols presents a complete consultant to the present matters in histopathology. With chapters on organ-based techniques with particular protocols for morphologic, molecular exam and pathological observations governing the healing administration of the ailments. Written within the hugely winning tools in Molecular Biology sequence structure, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols and tips about troubleshooting and warding off identified pitfalls.
Authoritative and useful, Histopathology: equipment and Protocols seeks to be an invaluable reference for pathologists, pathology citizens and fellows in addition to to the clinicians and scientists.
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Extra info for Histopathology: Methods and Protocols (Methods in Molecular Biology)
The most significant of these include: time of fixation, temperature, permeability, volume ratio, specimen dimension, pH and osmolality [2, 4, 5]. Recommendations for use and possible negative effects of each are summarized in Table 1. These factors, along with the appropriate choice of fixative, can significantly impact the ability to make an accurate diagnosis. The remainder of this chapter highlights the common fixatives, optimal use of each, and provides a resource for both pathologists and clinicians to ensure the appropriate fixative is selected for individual specimens.
19. 20. 21. transferred direct smears of the fine-needle aspirates. Am J Clin Pathol 139:754–758 Grizzle WE (2009) Special symposium: fixation and tissue processing models. Biotech Histochem 84(5):185–193 Kissel HD, Paulson TG, Liu K et al (2013) Feasibility of RNA and DNA extraction from fresh pipelle and archival endometrial tissues for use in gene expression an SNP arrays. Obstet Gynecol Int 2013:1–9 Wang J, Gouda-Vossos A, Dzamko N et al (2013) DNA extraction from fresh-frozen and formalin-fixed, paraffin-embedded human brain tissue.
Paraffin 30 min 13. Paraffin 30 min 14. Paraffin 30 min Processor: Thermo Scientific Excelsior™, no vacuum on any station, agitation on. Each step represents a subsequent station on the processor. Processor starts with 6 stations of 100 % reagent alcohol. The percentage of the alcohols is monitored by the processor and rotated based upon set criteria. Tissues: General surgical specimens 1. NBF 45 min + hold time (see Note 4) 2. NBF 1 h (see Note 5) 3. 75 % reagent alcohol 45 min (see Note 6) 4.
Histopathology: Methods and Protocols (Methods in Molecular Biology) by Christina E. Day