By Joanna Picot
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33. ABI Prism User Bulletin #2, ABI Prism 7700 Sequence Detection System, Dec 2001. Human Osteoblasts 53 34. 34 Beresford, J. , Graves, S. , and Smoothy, C. A. (1993) Formation of mineralised nodules by bone derived cells in vitro: a model of bone formation? Am. J. Med. Genet. 45, 163–178. 35. 35 Gundle, R. , Joyner, C. , and Triffitt, J. T. (1995) Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal cells. Bone 16, 597–601. 36. 36 Jones, S. , and Burnstock, G.
Osteoclasts form in culture medium supplemented with recombinant RANKL. Images are representative of cell appearance after the indicated number of days in culture using phase-contrast microscopy (all images to same scale). (A) D 0: cells are small, spherical and mononuclear. (B) D 7: cells are more spread out with larger cytoplasms but are still mononuclear. (C) D 14: cells are larger and begin to display an osteoclast-like appearance, some may be multinucleated. (D) D 21: cells are larger again, multinucleated, with an osteoclast-like appearance.
22 Rosenthal, N. (2003) Prometheus’s vulture and the stem cell promise. N. Engl. J. Med. 349, 267–274. 23. , Haynesworth, S. , Caplan, A. , and Bruder, S. P. (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell Biochem. 64, 295–312. 24. 24 Jones, E. , Kinsey, S. , et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthitis. Rheum. 46, 3349–3360. 25. 25 Fibbe, W. E. (2002) Mesenchymal stem cells: A potential source for skeletal repair.
Human Cell Culture Protocols by Joanna Picot