By W. F. Harrigan and Margaret E. McCance (Auth.)
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C. Select colonies by means of a replicator device such as the multipoint inoculator described by Reiter, Fryer and Sharpe (1965). 36 LABORATORY METHODS IN MICROBIOLOGY COMPOSITION OF CULTURE MEDIA A. Introduction A culture medium is any nutrient liquid or solid which can be used in the laboratory for the growth of micro-organisms. g. blood serum for animal pathogens, milk for milk micro-organisms, soil extract for soil micro-organisms). Whatever the medium, it must include all the necessary requirements for growth which vary according to the organism it is desired to grow but will include : 1.
This method is used in the demonstration of the presence of the obligate anaerobe Clostridium welchii in water or milk by the stormy-clot reaction, in which the medium is sterile milk rendered anaerobic and with a paraffin wax seal. Vaspar is a useful sealing compound prepared by melting together approximately equal amounts of petroleum jelly (Vaseline) and paraffin wax. E. The Anaerobic Jar This allows Petri-dishes or tubes containing any medium to be incubated in an oxygen-free atmosphere, and is necessary for the growth and purification of anaerobes by the use of streak plates.
It provides a convenient method of obtaining a representative sample when only a few colonies can be dealt with. The disc is constructed by first drawing a circle the size of a standard Petridish base, then drawing 2 diameters at right angles to each other, and 2 further diameters at angles of 9° to the first pair. The areas between the first diameters and those at 9° form four opposite sectors and constitute 1/10 of the total area. An ideal plate should contain about 30 colonies in this area, but in practice this number might be too great, so that this area is further divided into four.
Laboratory Methods in Microbiology by W. F. Harrigan and Margaret E. McCance (Auth.)