By Robert Weaver

ISBN-10: 0073525324

ISBN-13: 9780073525327

Molecular Biology, 4/e through Robert Weaver, is designed for an introductory path in molecular biology. Molecular Biology 5/e makes a speciality of the elemental ideas of molecular biology emphasizing experimentation. particularly writer, Rob Weaver, makes a speciality of the research of genes and their actions on the molecular point. in the course of the mix of fine illustrations and transparent, succinct writing scholars are offered primary molecular biology ideas.

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Extra info for Molecular Biology (5th Edition)

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17 contains a melting curve for DNA from Streptococcus pneumoniae. The amount of strand separation, or melting, is measured by the absorbance of the DNA solution at 260 nm. 3 Relative G + C Contents of Various DNAs Sources of DNA Dictyostelium (slime mold) Streptococcus pyogenes Vaccinia virus Bacillus cereus B. megaterium Haemophilus influenzae Saccharomyces cerevisiae Calf thymus Rat liver Bull sperm Streptococcus pneumoniae Wheat germ Chicken liver Mouse spleen Salmon sperm B. subtilis T1 bacteriophage Escherichia coli T7 bacteriophage T3 bacteriophage Neurospora crassa Pseudomonas aeruginosa Sarcina lutea Micrococcus lysodeikticus Herpes simplex virus Mycobacterium phlei Percent (G 1 C) 22 34 36 37 38 39 39 40 40 41 42 43 43 44 44 44 46 51 51 53 54 68 72 72 72 73 Source: From Davidson, The Biochemistry of the Nucleic Acids, 8th ed.

Jacobs, and others had demonstrated that RNA is composed of a sugar (ribose) plus four nitrogen-containing bases, and that DNA contains a different sugar (deoxyribose) plus four bases. They discovered that each base is coupled with a sugar–phosphate to form a nucleotide. We will return to the chemical structures of DNA and RNA later in this chapter. First, let us examine the evidence that genes are made of DNA. Transformation in Bacteria Frederick Griffith laid the foundation for the identification of DNA as the genetic material in 1928 with his experiments on transformation in the bacterium pneumococcus, now known as Streptococcus pneumoniae.

Separating the Two Strands of a DNA Double Helix Although the ratios of G to C and A to T in an organism’s DNA are fixed, the GC content (percentage of G 1 C) can vary considerably from one DNA to another. 3 lists the GC contents of DNAs from several organisms and viruses. The values range from 22–73%, and these differences are reflected in differences in the physical properties of DNA. When a DNA solution is heated enough, the noncovalent forces that hold the two strands together weaken and finally break.

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Molecular Biology (5th Edition) by Robert Weaver


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