By Zhanjiang (John) Liu
Fresh advancements in DNA marker applied sciences, specifically the emergence of unmarried Nucleotide Polymorphism (SNP) discovery, have rendered the various conventional equipment of genetic examine outmoded. subsequent iteration Sequencing and entire Genome choice in Aquaculture comprehensively covers the present nation of analysis in complete genome choice and applies those discoveries to the aquaculture particularly. The textual content starts off with a radical assessment of SNP and transitions into issues resembling subsequent iteration sequencing, EST facts mining, SNP caliber review, and full genome choice ideas. finishing with a dialogue of the technology's particular functions to the undefined, this article will be a priceless reference for these interested by all facets of aquaculture research.Special Features: Unique linking of SNP applied sciences, subsequent new release sequencing applied sciences, and full genome choice within the context of aquaculture researchThorough overview of unmarried Nucleotide Polymorphism and current research8-page colour plate part that includes unique illustrations
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1). The most popular approach for the analysis of CNV is the array-based CGH (array CGH or a-CGH). Array CGH is also called molecular karyotyping. , 2005). It is a hybridization-based approach using array as a platform. The use of array allowed for the placement of a large number of features (target sequences, sometimes also referred to as probes; but we will use features to avoid confusion with hybridization probes), which in this case are short sequence oligos based on the reference genome sequence.
After hybridization, the ratio of the fluorescence intensity of the test probe to that of the reference probe is calculated. The ratio, upon calibration, reveals the copy number differences between the genomes. The hybridization result can be measured using microarray scanner. Then the feather extraction software can be used to quantify the hybridization images. Finally, the test file outputs are used to do the CNV analysis using CNV detection software. 2 An example of using array CGH for the detection of chromosomal segment duplications in cancer.
The ratio, upon calibration, reveals the copy number differences between the genomes. The hybridization result can be measured using microarray scanner. Then the feather extraction software can be used to quantify the hybridization images. Finally, the test file outputs are used to do the CNV analysis using CNV detection software. 2 An example of using array CGH for the detection of chromosomal segment duplications in cancer. See color insert. , 2004). In many cases of cancers, the malignant genome is instable, and segmental duplications can happen in certain genomic regions depending on the cancer type.
Next Generation Sequencing and Whole Genome Selection in Aquaculture by Zhanjiang (John) Liu