By Kivie Moldave, Lawrence Grossman

ISBN-10: 0121818837

ISBN-13: 9780121818838

The severely acclaimed laboratory ordinary, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. on the grounds that 1955, each one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - really a vital booklet for researchers in all fields of existence sciences

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Additional resources for Part C: Nucleic Acids and Protein Synthesis

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Atomic Energy Commission u n d e r contract with Union Carbide Corporation. 2,]. F. Weiss, R. L. Pearson, and A. D. Kelmers, Biochemist U 7, 3479 (1969). aL Shugart, B. Chastain, and G. D. Novelli, Biochim. Biophys. Acta 186, 384 (1969). 36 TRANSFER RNA [3] e n z y m e p r o t e i n 4"5 in a final v o l u m e o f 1 ml. F o r m e t h i o n y l a t i o n , 8 n m o l e s o f L-[14C]methionine (New E n g l a n d N u c l e a r C o r p o r a t i o n ; spec. , 217 /zCi//zmole) a r e a d d e d ; a n d for f o r m y l a t i o n , 8 n m o l e s o f L-[lZC]methionine a n d 2 n m o l e s o f enzymatically synthesized [ m e t h e n y l 14C]5"~°-methenyltetrahydrofolate6"7 are included.

Jacobson, P,vc. Nat. Acad. Sci. U. S. 62, 1137 (1969). rC. R. Merril, Biopolymers 6, 1727 (1968). SK. Takeishi, S. Nishimura, and T. Ukita, Biochim. Biophys. Acta 145, 605 (1967). tG. Dirheimer and B. Kuntzel, Bull. Soc. Pharm. Strasbourg 9, 97 (1966). UM. P. Stulberg, K. R. Isham, and A. Stevens, Biochim. Bioplo's. Acta 186, 297 (1969). ~J. L. Epler, Biochemistry 8, 2285 (1969). wW. E. Barnett, C. J. Pennington, and S. A. Fairfield, Proc. Nat. Acad. Sci. U. , 63, 1261 (1969). ~B. S. Dudock, G.

4 mg/ml bentonite, and then mixed with 30 ml of the buffer-salt solution listed above. Fifty milliliters of freshly prepared 80% phenoP 3 in the same solution is added and the mixture is shaken at 4 ° for 30 minutes. After centrifugation, 14 11Fifteen percent glycerol in the medium protects certain mammalian synthetases from inactivation d u r i n g preparative procedures, similar to that found with E. coli synthetases [K. H. Muench and P. Berg, in "Procedures in Nucleic Acid Research" (G. L. Cantoni and D.

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Part C: Nucleic Acids and Protein Synthesis by Kivie Moldave, Lawrence Grossman

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